Cell Phenotypes in human Amniotic Fluid
نویسندگان
چکیده
منابع مشابه
Cell Phenotypes in Human Amniotic Fluid
Stem cells capable of long-term proliferation and differentiation into different cell types may be a promising source of cells for regenerative medicine. Recently, much attention has been paid to fetal stem cells, among which are cells from amniotic fluid (AF). We have isolated amniotic stem cells from 3 AF samples. Flow cytometry, RT -PCR and immunohistochemistry have shown that these cells ex...
متن کاملAmniotic fluid cell cultures.
In an 18-month period, 249 samples of amniotic fluid were used experimentally in attempts to enhance success in cell cultures. None ofthe experimental situations proved entirely satisfactory in this aim. Emphasis is laid on the quality ofserum used in culture. Particularly satisfactory serum should be retained for use in amniotic fluid cell cultures which are dependent on unknown growth stimula...
متن کاملHuman papillomavirus in amniotic fluid
BACKGROUND There is evidence to suggest that human papillomavirus (HPV) can cross the placenta resulting in in-utero transmission. The goal of this study was to determine if HPV can be detected in amniotic fluid from women with intact amniotic membranes. METHODS Residual amniotic fluid and cultured cell pellets from amniocentesis performed for prenatal diagnosis were used. PGMY09/11 L1 consen...
متن کاملDifferentiation in human amniotic fluid cell cultures: I: Collagen production.
The collagen produced by differentiated cells cultured from human amniotic fluid was characterized in two ways. By chain composition and by 4-hydroxyproline:3-hydroxyproline isomer ratio, the collagen synthesized by F-type (fibroblast) cells was indistinguishable from that made by cultured fetal dermal fibroblasts. The predominant cells in young amniotic fluid cultures, termed AF-type, produced...
متن کاملObservations on human amniotic fluid cell strains in serial culture.
Observations made on 31 amniotic fluid cell strains serially cultured until senescent are recorded. The cell strains had an average life in culture of 13-9 passages (range 3-29). The source of the amniotic fluid from which the cultures were initiated did not influence the behaviour of the cell strains. The behaviour ofthe cell strains was unrelated to the growth characteristics of the primary c...
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ژورنال
عنوان ژورنال: Acta Naturae
سال: 2009
ISSN: 2075-8251
DOI: 10.32607/20758251-2009-1-2-98-103